Coding

Part:BBa_K2486102:Design

Designed by: Tiago Lubiana Alves   Group: iGEM17_USP-Brazil   (2017-10-27)


Anti-malarial single-chain antibody m1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No stop codon added facilitating fusion of secretion tag.


Source

Derived from mouse monoclonal antibodies. Can be synthesized.

References